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Ligationen fest im Griff: Eine wichtige Einschränkung der Protein‐Semisynthese ist der Bedarf eines Cys an der Ligationsstelle in nativen chemischen Ligationsreaktionen. Eine Transferase wird nun beschrieben, die Homocystein an den N‐Terminus eines exprimierten Proteins knüpfen kann (siehe Schema). Homocystein kann für eine Ligationsreaktion genutzt und dann in Met umgewandelt werden. Dies erlaubt...
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