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α‐l‐Fucosidases are key enzymes for the degradation of intestinal glycans by gut microbes. In this work, three putative α‐l‐fucosidases (Afc1, Afc2, and Afc3) genes from Clostridium perfringens ATCC 13124 were cloned and expressed in Escherichia coli. Afc1 had the α‐l‐fucosidase domain of glycoside hydrolase (GH) 29 family but showed no enzyme activity toward all the substrates examined. The putative...
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