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Using lactose as the substrate, galacto-oligosaccharides containing β-d-galactose residues were synthesised with β-galactosidase from Lactobacillus bulgaricus L3. The reaction mixture was fermented by yeast cells to consume the monosaccharides and disaccharides, and then it was fully acetylated in the presence of acetic anhydride under I 2 catalysis. Column chromatography of the resulting...
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