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The first component of the mitochondrial electron transport chain is respiratory complex I. Several high‐resolution structures of complex I from different species have been resolved. However, despite these significant achievements, the mechanism of redox‐coupled proton pumping remains elusive. Here, we combined atomistic docking, molecular dynamics simulations, and site‐directed mutagenesis on respiratory...
We determined the structure of a complete, dimeric F1Fo-ATP synthase from yeast Yarrowia lipolytica mitochondria by a combination of cryo-EM and X-ray crystallography. The final structure resolves 58 of the 60 dimer subunits. Horizontal helices of subunit a in Fo wrap around the c-ring rotor, and a total of six vertical helices assigned to subunits a, b, f, i, and 8 span the membrane. Subunit 8 (A6L...
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