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Signal transduction ATPases with numerous domains (STAND) are widespread proteins, whose activation involves inducer‐dependent conversion of resting ADP‐bound monomers into active ATP‐bound multimers. This process notably comprises opening of the nucleotide‐binding oligomerization domain (NOD), nucleotide exchange and NOD‐mediated multimerization. How inducer binding to the sensor domain, whose structure...
MalK, the cytoplasmic component of the maltose ABC transporter from Escherichia coli is known to control negatively the activity of MalT, the activator of the maltose regulon, through complex formation. Here we further investigate this regulatory process by monitoring MalT activity and performing fluorescence microscopy analyses under various conditions. We establish that, under physiological conditions,...
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