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A method for quantifying a substrate having improved measurement accuracy and measurement sensitivity is disclosed. A constant voltage is applied to an electrode system, between a working electrode and a counter electrode, via a resistor connected in series with the electrode system, so as to oxidize an electron acceptor which has been reduced by an enzyme reaction of the substrate, thereby determining...
The biosensor of this invention can quantify a substrate in a sample liquid by electrochemically measuring the amount of an electron acceptor that has been reduced by electrons generated in a reaction between the substrate and an oxidoreductase. The biosensor has an electrically insulating substrate and an electrode system formed on the substrate including a working electrode, a counter electrode...
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