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Previous work has shown that phorbol esters modulate chemotaxis. Here, we demonstrate that PKC activation via phorbol 12-myristate 13-acetate (PMA) treatment of MDA-MB-231 cells inhibits EGF-induced cell spreading, the initial event of motility and chemotaxis. Of five PKC isoforms (α,ι,λ,δ,andε) identified in this cell line, PMA treatment only induced PKCα translocation from the cytosol to the membrane,...
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