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A high pH anion exchange chromatographic (HPAEC) system for the separation of isomeric sialo-oligosaccharide products was developed. Employing this system, using Galβ1->4GlcNAcβ1->2Manα1->6Manβ1->4GlcNAc as a substrate, a Galβ1->4GlcNAc-R α2->3-sialyltransferase activity was detected for the first time in human liver. This activity is expressed together with the prevalent α2->6-sialyltransferase...
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