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A combined UPLC–tandem mass spectrometric (UPLC–MS/MS) technique has been validated for quantitation of protein free efavirenz (EFV) as well as total concentrations of EFV in human blood and seminal plasma. The analytical method possesses capabilities for concentration measurements of EFV ranging from 0.5 to 10,000ng/ml with an accuracy (%dev) of −5.2–8.0% and precision (%CV) of <8%. Standard curves...
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