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Off-line two-dimensional reversed-phase liquid chromatography (2D-RPLC) coupled to electrospray ionization-ion trap mass spectrometry (ESI-ITMS) was operated in positive mode (PI) to characterize polymethoxylated flavonoids (PMFs) in botanical sample. The fragments of [M+H−n×15] + produced by loss of one or more methyl group from the protonated molecules, as well as [M+H−14] + , [M+H−29]...
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