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For the first time, a β-1,3-glucanase was isolated from the gut of sea cucumber. The enzyme was purified to homogeneity by ammonium sulfate precipitation, ion exchange chromatography with diethylaminoethyl (DEAE)-Sepharose CL-6B, hydrophobic interaction chromatography with Butyl-Sepharose and preparative electrophoresis with polyacrylamide gel electrophoresis (PAGE). The molecular weight of the purified...
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