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A 10 nm nanogold particle was used to label monoclone CEA antibody to prepare an immunonanogold resonance scattering probe (AuCEAAb) for detection of CEA. In the pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of PEG-6000, the immunoreaction between CEA and AuCEAAb took place to form the immunonanogold complex that can be removed by centrifugation technique. The supernatant containing AuCEAAb...
A strain of denitrifying bacterium DN7, a facultative aerobic bacterim, was isolated from the sea area near the Changhai County of Dalian. Some physiological and biochemical properties were characterized, 16S rDNA gene sequence was amplified by PCR, and its nucleotide sequence was determined. Based on its physiological and biochemical properties, homology and phylogenetic analysis, strain DN7 was...
Strain PSB-01, a bioactive marine photosynthetic bacterium, was isolated from marine sediments of the Bohai Sea Area. Cells are ovoid to short rod-shaped, 0.3-0.5 mum in diameter, and 1.0-1.5 mum in length. Multiply by binary fission, gram-negative and contain internal photosynthetic membrane systems in which the photosynthetic pigments bacteriochlorophylla and carotenoids are located. Researches...
Love-mode immunosensors based on ZnO/36degYX-LiTaO3 structures are studied. The ZnO films are grown on the 36degYX-LiTaO3 substrates by RF magnetron sputtering technique. The crystalline structures of the ZnO films are investigated by the X-ray diffraction (XRD) analysis, which show that the highly orientated (002) films are obtained. Then the Love-mode sensors are fabricated for monitoring antibody-antigen...
In this paper, a kind of Love wave sensors with SiO2/36degYX LiTaO3 structures for monitoring antibody-antigen immunoreactions in aqueous solutions in real time is presented. In this study, the devices with low insertion losses are achieved when the devices are in direct contact with the solutions. In the immunosensing measurements, a goat anti human-immunoglobulin G (HigG) is immobilized on the device...
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