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Interleukin-4 (IL-4) is a pleiotropic cytokine with broad spectrum of biological effects on target cells. This study deals with the mammalian expression of canine IL-4 (cIL-4) in canine articular chondrocytes (CAC) and its ability to down-regulate pro-inflammatory cytokines, enzyme mediators and their catabolites. We transfected cIL-4 in CAC which were then stimulated with canine recombinant IL-1β...
Human recombinant IL-1β and TNFα have been previously used to induce a cytokine response in canine chondrocytes. In order to establish this functional relation in a homologous system in vitro, we have developed both 2D and 3D models of inflammatory arthritis using canine recombinant cytokines in canine articular chondrocytes. IL-1β and TNFα were cloned and subsequently expressed in Escherichia coli...
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