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Two reactions were studied with three varieties of starch granules from maize, wheat, and rice. In Reaction-I, the granules were reacted with 1mM ADP-[ 14 C]Glc and in Reaction-II, a portion of the granules from Reaction-I was reacted with 1mM ADP-Glc. The starch granules were solubilized and reacted with the exo-acting glucoamylase and beta-amylase to an extent of 50% or less of the 14...
Two mechanisms are recognized for polysaccharide chain elongation: (a) the nonreducing-end, primer-dependent mechanism and (b) the reducing-end, two-site insertion mechanism. We recently demonstrated the latter mechanism for starch biosynthesis by pulsing starch granules with ADP-[ 14 C]Glc and chasing with ADPGlc for eight varieties of starch granules. Others have reported the addition of...
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