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A suspension culture of bromegrass (Bromus inermis Leyss cv. Manchar) provides a unique system for studying cold hardiness mechanism, where freezing tolerance can be independently induced by two different stimuli, cold and exogenous abscisic acid (ABA). Freezing tolerance was induced in bromegrass cells by exposure to low temperatures for 7 days (LT50: −8.1 °C) and also by incubation with ABA for...
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