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This study reports a novel, simple and sensitive immunoassay using fluorescence quenching caused by gold nanoparticles coated with antibody. The method is based on a non-competitive heterogeneous immunoassay of human IgG conducted by the typical procedure of sandwich immunocomplex formation. Goat anti-human IgG was first adsorbed on polystyrene microwells, and human IgG analyte was captured by the...
In this paper, a new immunosensor has been proposed based on the receptor protein adsorbed directly on a porous gold film. The film was produced electrochemically on a glassy carbon electrode in 0.08M hydrogen tetrachloroaurate solution containing 0.004M lead acetate with applied potential of −0.5V (versus Ag/AgCl) for 50s. The assay was carried out based on a sandwich procedure. The impedance signals...
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