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A competitive indirect time-resolved fluoroimmunoassay (TRFIA) for detecting aflatoxin M1(AFM1) contamination in milk was developed, by using aflatoxin M1-bovineserum albumin conjugate, anti-AFM1 antibody, and Eu-labeled goat anti-rabbit antibody. To improve the sensitivity of the assay, the concentrations of the coating antigen and anti-AFM1 antibody were varied to optimize the condition of the immunological...
The objective of this study is to establish a highly sensitive, time-resolved fluoroimmunoassay (TRFIA) for the rapid detection of chlorpromazine (CPZ). The antigen CPZ-OVA was used to coat a microtiter plate to compete with free chlorpromazine for binding to the anti-chlorpromazine monoclonal antibody. Europium (Eu3+)-labeled goat anti-mouse antibody was used as the tracer. The labeling rate of Eu...
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