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The structure, phylogeny and in vivo function of the base pair formed between nucleotides 32 and 38 of the tRNA anticodon loop are reviewed. The A32–U38 pair, which is highly conserved in tRNA 2Ala and sometimes observed in tRNA 2Pro , was recently found to decrease the affinity of tRNAs to the ribosomal A site relative to other 32–38 combinations. This suggests that the...
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