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We report a validated method for the determination of extra- and intracellular [1- 13 C]-α-ketoisovalerate ([1- 13 C]-KIV) enrichments by gas chromatography-mass spectrometry. Standardization curves were prepared by enzymatic oxidation of [1- 13 C]-valine enriched standards of known composition. Slopes of [1- 13 C]-valine standardization curves (mean+/-SD:...
A gas chromatographic–combustion isotope ratio mass spectrometric (GC–C-IRMS) method for the determination of [1- 13 C]valine enrichments in protein hydrolysates is described. Using a quick derivatization method, δ 13 C values of the N-methoxycarbonyl methyl ester of valine can be determined from baseline separated GC peaks. Evaluation studies with respect to precision, accuracy, linearity,...
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