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The influence of transglutaminase (TG) treatment on the changes in the physico-chemical properties of reconstituted skim milk (10 wt%) with different amounts of added ethylenediaminetetraacetic acid (EDTA) (0–50 mM) was studied. The partition of salts (Ca, Mg, K, Na and inorganic phosphate) was determined experimentally by atomic absorption and emission spectroscopy, colorimetry and 31P-nuclear magnetic...
Phosphoproteins and phosphopeptides were expressed by E. coli to give yields of 30–200mg of purified protein per litre with an average degree of phosphorylation at multiple sites of 61–83%. The method employed two compatible cohabiting plasmids having low and high copy number, expressing a protein kinase and, more abundantly, the substrate (poly)peptide, respectively. It was used to phosphorylate...
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