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A new full-length cDNA (Ssd12) encoding a Δ12-fatty acid desaturase (Δ12-FAD) was cloned from Sapium sebiferum using RT-PCR and RACE methods. Ssd12 contained a 1146 bp open reading frame encoding a protein of 381 amino acids. The amino acid sequence showed a much higher match with microsomal Δ12-FAD amino acid sequences than chloroplast Δ12-FAD amino acid sequences. Genomic Southern blot analysis...
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