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An immobilized trypsin reactor that was based on aptamers has been developed and applied for the first time for proteomic digestion. Briefly, 25 single-stranded DNA aptamers that were specific for trypsin were obtained after SELEX (systematic evolution of ligands by exponential enrichment) selection. TApt.23 (no. 23 trypsin aptamer), which had the lowest dissociation constant (Kd) value (0.0123μM),...
The aim of this study was to investigate the potential of poly(acrylic acid)–cysteine (PAA–cys) solution and microparticles to enhance the transport of vitamin B12 (VB 12) across Caco-2 cell monolayer and rat intestinal mucosa. Thiolated PAA was synthesized by covalent attachment of l-cysteine. Microparticles were prepared by spray-drying and characterized regarding their size, morphology, thiol group...
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