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Perfusion cell culture processes allow the steady‐state culture of mammalian cells at high viable cell density, which is beneficial for overall product yields and homogeneity of product quality in the manufacturing of therapeutic proteins. In this study, the extent of metabolic steady state and the change of the metabolite profile between different steady states of an industrial Chinese hamster ovary...
In this work, two external filtration devices for cell retention, the commercial alternating tangential flow (ATF) system and a tangential flow filtration (TFF) setup driven by a bearingless centrifugal pump were compared. Physical characterization of the bioreactor hydrodynamics revealed significantly smaller maximum stress values in the ATF at similar mixing and oxygen mass transfer rates. Suitable...
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