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In this study, we developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay for Salmonella detection targeting the gene62181533. The addition of manganous ions and calcein to the reaction system allowed the visualization of an apparent color change during the amplification reaction. The detection limit of LAMP using a six-primer combination was 1.057 copies/μl (standard plasmid),...
Listeria monocytogenes, Staphylococcus aureus, Escherichia coli O157, Salmonella spp. and Bacillus cereus are major food-borne pathogens that often contaminate food and cause outbreaks of illness. This investigation determined the incidence of the five pathogens in commercial cold dishes food. A total of 99 samples, consisting of meat, vegetable and soy products were collected and analyzed using conventional...
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