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In the present study, we obtained the full‐length cDNA sequences of TLR21 and TLR22 from Nile tilapia (Oreochromis niloticus) (OnTLR21 and OnTLR22, respectively). The gene structure and expression patterns of OnTLR21 and OnTLR22 were also studied in order to understand the function of these genes in tilapia immunity. The OnTLR21 gene was 3196 bp in length, with an open reading frame (ORF) of 2940 bp...
Chromosomal karyotypes of Oreochromis mossambicus and O. urolepis hornorum and their hybrid were analysed by means of Cot‐1 DNA bandings through fluorescence in situ hybridization (FISH). To identify all chromosomes, Cot‐1 DNA – which contains highly and moderately repetitive DNA – was extracted from genomic DNA, labelled as a probe with Dig‐11‐dUTP, and in situ hybridized to spreads of mitotic chromosomes...
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