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The purpose of this study was to establish a suitable 2-dimensional polyacrylamide gel electrophoresis (2-DE) system for proteomics analysis in Brassica napus. Proteins of rapeseed variety 08127 were separated in gel brands (pH 4–7) using trichloroacetic extraction method under the improved isoelectric focusing (IEF) procedure. A clean 2-DE map was obtained, indicating that the system was effective...
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