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To develop the technology for CO2 sequestration, the free and immobilized carbonic anhydrase treatment was performed. The amount of dissolved CO2 was increased by 3-fold after enzyme treatment of 5 ppm, and the amount of calcium carbonate increased linearly as the CaCl2 was increased to 100 mM. To immobilize the carbonic anhydrase, an aluminum oxide carrier surface was functionalized with octadecyltrichlorosilane,...
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