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We have developed an optical assay for NADH (Dihydronicotinamide adenine dinucleotide) based on the catalytic growth of gold–silver core–shell nanoparticles (Au–Ag–CSNPs). The nanoparticles were immobilized on pretreated glass slide and are shown to catalyze the NADH-mediated reduction of Ag(I) ions in the presence of 1,4-benzoquinone and cetyltrimethyl ammonium ion. This leads to the formation of...
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