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The aim of this study was to evaluate sperm fertilization rates and in vitro embryo development rates for sexed and non-sexed semen selected using a silane-coated silica colloid method (Isolate) or Percoll. Frozen/thawed, sexed and unsexed semen samples from four Holstein bulls were randomly allocated to one of two different density gradient selection methods. Sperm quality (motility, concentration,...
A series of experiments were designed to study factors affecting the ability of pig spermatozoa to fertilize in vitro and the rate of development of pig oocytes fertilized in vitro.In experiment 1, the effect of different capacitation promoters on the ability of spermatozoa to fertilize pig oocytes in vitro was studied. Fresh pig ejaculated spermatozoa were preincubated in m-TCM199 without bovine...
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