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Pseudomonas putida GP01 cells that had accumulated medium-chain-length polyhydroxyalkanoates (PHAMCL) secreted 3-hydroxyoctanoate and 3-hydroxyhexanoate when incubated in alkaline buffers. The release of acids strongly decreased the pH resulting in less efficient secretion of 3HAMCL at neutral pH. To increase the yield of secreted MCL-hydroxyalkanoates, experiments at constant pH in a pH stat apparatus...
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