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EF-1a binds aminoacyl-tRNA to the ribosome with the hydrolysis of GTP; the βγδ complex facilitates the exchange of GDP for GTP to initiate another round of elongation. To examine the subunit structure of EF-1 and phosphorylation by protein kinase CKII, recombinant β, γ, and δ subunits from rabbit were expressed in E. coli and the subunits were reconstituted into partial and complete complexes and...
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