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Artificial chaperone consists of a detergent (cetyltrimethylammonium bromide, CTAB) for binding denatured protein and a stripper (β-cyclodextrin) for the release of the denatured protein from the detergent–protein complex. The authors have proposed artificial chaperone (AC)-assisted immobilized metal affinity chromatography (AC-IMAC) for protein refolding and purification, and have herein studied...
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