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The hydrazinolysis of S‐cyanylated peptide provides an alternative way to afford protein α‐hydrazide, a key reagent used in native chemical ligation (NCL), without the aid of any inteins or enzymes. The currently used non‐selective S‐cyanylation, however, allows no other cysteine in the protein besides the one at the cleavage site. Herein, we report a regioselective S‐cyanylation and hydrazinolysis...
The hydrazinolysis of S‐cyanylated peptide provides an alternative way to afford protein α‐hydrazide, a key reagent used in native chemical ligation (NCL), without the aid of any inteins or enzymes. The currently used non‐selective S‐cyanylation, however, allows no other cysteine in the protein besides the one at the cleavage site. Herein, we report a regioselective S‐cyanylation and hydrazinolysis...
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