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Three different urea-resistant Staphylococcus aureus V8 protease derivatives (V8Δ53-U1, V8Δ53-U5 and V8Δ53-U8) were obtained by random mutagenesis using a polymerase chain reaction (PCR) method. From DNA sequence analysis of the mutants, three different amino acid substitutions, D44E, N71S and K147R, were identified. The half-lives of these mutant enzymes were 3 to 5 times longer than that of the...
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