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In humans and animals, intestinal flora is indispensable for bile acid transformation. The goal of our study was to establish gnotobiotic mice with intestinal bacteria of human origin in order to examine the role of intestinal bacteria in the transformation of bile acids in vivo using the technique of gnotobiology. Eight strains of bile acid-deconjugating bacteria were isolated from ex-germ-free mice...
To develop a new simple method for identification of bacteria in the Bacteroides subgroup isolated from human feces, we designed a panel of four 16S rDNA-targeted oligonucleotide probes specific for each cluster of the Bacteroides subgroup. The probes Bac and bacvul were targeted to the Bacteroides cluster, and the probes Pre and Por have their target regions characteristic to those of the Prevotella...
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