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A truncated cDNA of the human macrophage colony-stimulating factor gene, encoding amino acid residues from 3 to 149 of the native M-CSF, was isolated by using the polymerase chain reaction and inserted into plasmid pET3d. It directs the expression of truncated M-CSF fused with a six-histidine tail peptide in E. coli BL21 (DE3) LysE under the control of T 7 promoter. The expression level...
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