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The interaction between protein and soluble organic matter is studied through layer-by-layer assembly of lysozyme (LSZ) and purified Aldrich humic acid (PAHA) at a solid surface (2-D) and in solution (3-D). By bringing a silica surface in alternating contact with solutions of LSZ and PAHA a layer-by-layer LSZ–PAHA assembly is formed. At pH 5 the negative charge density of PAHA is about 3 times that...
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