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BackgroundA lab‐scale anaerobic‐anoxic/nitrification (A2N) two‐sludge sequencing batch reactor (SBR) system was operated to assess the relationship among intracellular polymer conversions, bacterial population dynamics and nutrient removal performance in response to the varying influent COD/N/P.
ResultsComplete nutrient removal was achieved in the A2N‐SBR in Run 3 where the COD/P and COD/N were 20.6 and 6.9, respectively. This coincides well with the highest P release/HAc uptake ratio occurring in Run 3, confirming high abundance of denitrifying phosphorus accumulating organisms (DPAOs) in the biomass. Under the stress of N or P limitation, more poly‐β‐hydroxyalkanoates (PHA) was synthesized, with the increasing proportion of poly‐hydroxyvalerate (PHV) in PHA. The population biodiversity decreased with increasing carbon loading when P loads were maintained at constant levels, and recovered to the initial level after P loading decreased...
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