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Sedimentation equilibrium measurements and scanning transmission electron microscopy (STEM) mass mapping of the extracellular, hexagonal bilayer hemoglobin (HBL Hb) of the earthwormLumbricus terrestrisprovided masses of 3.41 to 3.66 MDa and 3.56 (+/-0.13) MDa, respectively. The Hb also contains 57.2 (+/-6.0) moles of tightly bound Ca per mole of protein. The Hb and its subunits obtained by dissociation,...
Electrospray mass spectrometry has been used to measure the masses of the species present in solutions of three serine proteases (α-chymotrypsin, subtilisin Carlsberg and subtilisin BPN') before, during and after completion of the hydrolytic reaction with cinnamoyl imidazole and indole acryloyl imidazole. The masses measured during the reaction demonstrated that covalent O-acyl enzyme intermediates...
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