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In this study, a method was developed and validated for the quantification of metolazone in human plasma samples. This method involves high-performance liquid chromatography coupled with tandem mass spectrometry and is more sensitive, selective and rapid than currently available methods. Chromatography was performed using a Phenomenex® Luna C18 column (100 mm × 2.0 mm, 5 μm, 100 Å) with an isocratic...
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