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Pyrodictium occultum is a hyperthermophilic archaeum that grows optimally at 105 o C. To study how tRNA molecules in P occultum are thermally stabilized, we isolated the initiator tRNA gene from the organism using a synthetic DNA probe of 74 bp containing the known nucleotide sequences that are conserved in archaeal initiator tRNAs. A HindIII fragment of 700 bp containing thePyrodictium...
Cytoplasmic tRNA Asp of rat liver was purified by the solid-phase hybridization method and its nucleotide sequence was analyzed by Donis-Keller's method. The results suggested that the two nucleotides next to the anticodon were identical to its gene sequence, a finding that is inconsistent with a previous report demonstrating by several methods that C32 and T33 on the tRNA A...
The ability of bovine mitochondrial tRNA Met with the anticodon f 5 CAU (where f 5 C is 5-formylcytidine) to decode AUG and AUA codons was examined in a codon-dependent ribosomal binding assay. The AUG codon stimulated the binding of Met-tRNA Met to mitochondrial ribosomes in the presence of EF-Tu/Ts mt . In contrast, the AUA codon...
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