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One of the most exciting areas of cell biology during the last decade has been the study of lipid droplets. Lipid droplets allow cells to store non-polar molecules such as neutral lipids in specific compartments where they are sequestered from the aqueous environment of the cell yet can be accessed through regulated mechanisms. These structures are highly conserved, appearing in organisms throughout...
Eukaryotic cells store cholesterol/sterol esters (SEs) and triacylglycerols (TAGs) in lipid droplets, which form from the contiguous endoplasmic reticulum (ER) network. However, it is not known if droplets preferentially form from certain regions of the ER over others. Here, we used fission yeast Schizosaccharomyces pombe cells where the nuclear and cortical/peripheral ER domains are distinguishable...
Cells sequester neutral lipids in bodies called lipid droplets. Thus, the formation and breakdown of the droplets are important for cellular metabolism; unfortunately, these processes are difficult to quantify. Here, we used time‐lapse confocal microscopy to track the formation, movement and size changes of lipid droplets throughout the cell cycle in fission yeast Schizosaccharomyces pombe. In theory,...
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