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Genetic transfer and regulation methodologies in vivo are essential for analysis and manipulation of cells and tissue functions. A variety of such systems have also been applied to basic and preclinical studies on gene therapy and regenerative medicine, etc., while clinical utilization has not been successful enough so far. The gene transfection efficiencies of nonviral vectors are drastically improved...
Genetic transfection is a fundamental technology required for analysis and control of cells and tissues. The efficiency of gene transfection is drastically improved by using Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) gene and oriP, which are derived from EBV genome, as components of plasmid vectors to be combined with various non-viral gene transfer vehicles. By means of the multiscale manipulation...
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