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•We evaluated the detection limits for a polymerase chain reaction assay targeting travelers' diarrhea pathogens. •Whatman™ FTA Elute cards were smeared with spiked stool for testing. •The limit of detection ranged from 102 to 105CFU, PFU, or cysts/g for most pathogens. •The limit of detection for Campylobacter and norovirus increased with prolonged storage. •Cryptosporidium was poorly detected...
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