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The ionic liquid, 1-butyl-3-methylimidazolium methylsulfate ([BMIM][MeSO4]), was used to investigate the catalytic mechanism of horseradish peroxidase (HRP). The ionic liquid decreased both Km and kcat values for the HRP-catalyzed oxidation of guaiacol (2-methoxyphenol) by H2O2. These studies imply that [BMIM][MeSO4] inhibits the enzyme in an uncompetitive manner. The incorporation of substrate stabilization...
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