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An expressed peptide was proven to be useful as a building block in the synthesis of a polypeptide via the thioester method. A partially protected peptide segment, [Lys(Boc) 15,25,31,48,57,68,80,95 ]-Max(14-101), was prepared via transamination of an N-terminal amino group from p21Max(13-101),...
Reaper was synthesized by condensing two peptide segments, one of which was a partially protected peptide thioester, Boc-[Lys(Boc) 20 ]-reaper(1--32)-SCH 2 CH 2 CO-β-Ala-NH 2 , and the other a peptide which contained an Cys(Acm) residue, H-[Cys(Acm) 49 , Lys(Boc) 52,56,59,62 ]-reaper(33--65)-OH...
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