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The ECM25 deletion mutant of industrial brewing yeast, G03/a, was constructed by replacing the ECM25 gene with the kanMX gene. The transformant was verified to be genetically stable. The PCR analysis showed that ECM25 gene in the G-03/a was deleted. Under aerobic conditions of 11°C and 28°C, compared with the host strain G-03, the excretive glutathione concentration of G-03/a increased by 21.4% and...
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