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Due to its variety of functions, rhubarb has been used for thousands of years in many countries. It is commonly used after processing. Processing usually affect the chemical profile and the contents of active compounds in herbals, leading to changes of their bioactivities. Here, an approach of metabolite profiling and diagnostic ion filtering strategy with liquid chromatography–quadrupole/time-of-flight...
An endo-polygalacturonase gene (pg I) was cloned from Penicillium sp. CGMCC 1669 and expressed in Pichia pastoris. The full-length cDNA consists of 1140bp and encodes a glycoside hydrolase family 28 protein with a calculated molecular mass of 37.5kDa. The optimal pH and temperature of purified recombinant endo-PG I were 3.5 and 40°C, respectively. At 0°C, endo-PG I still retained 7.3% of the maximal...
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