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Tissue engineered skin constructed by cultured seed cells provides a new way to accelerate skin wound healing and improve the quality of tissue repair. Conventional models with dermal scaffold material based on collagen hydrogels, however, have poor stability and unsatisfactory longevity. Here, we describe an improved acellular amniotic membrane as a scafford with epidermal stem cells for tissue engineering...
The amniotic membranes have unique properties including transparency, stimulatory effect on cell proliferation and lack of immunogenicity, which may have clinical utility for cell delivery. In this chapter, we investigate the possibility of amniotic membranes loaded with bone marrow mesenchymal stem cells for healing of deep burn wound. The mesenchymal stem cells derived from human bone marrow were...
It is well known that the telomerase is present at high levels in embryonic tissues, which plays an important role in replication and proliferation capacity of the stem cells. But the telomerase activity in cultured epidermal stem cells derived from human skins is not fully understood. In this chapter, we focus on the isolation, culture and identification of the epidermal stem cells derived from human...
Epidermal stem cells have the characteristic of the undifferentiated cells in the morphology, and are the biological source of the skin and its subsidiary in the development, repair and reconstruction. The proliferation and differentiation of epidermal stem cell are influenced by many factors, such as integrin, signal transduction pathway and cytokines. Current researches have not yet been able to...
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