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Amyloid-β peptide (Aβ), a putatively causative agent of Alzheimer’s disease (AD), is proteolytically derived from β-amyloid precursor protein (APP). Here we describe cellular assays to detect the activity of the key protease β-site of APP cleaving enzyme 1 (BACE1) based on an artificial reporter construct containing the BACE1 cleavage site of APP. These methods allow identification of inhibitors and...
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